Rapid Electrophoretic Staining and Destaining of Polyacrylamide Gels

Rapid Coomassie blue staining and destaining of polyacrylamide gels.

Staining acidic phosphoproteins (phosvitin) in electrophoretic gels.

The principal phosphoglycoproteins of avian and amphibian egg yolk, known as phosvitins, have been examined extensively in developmental studies of estrogenic induction of protein synthesis (1) and, more recently, as substrates for protein kinases which phosphorylate the basic nuclear proteins (2,3). Phosvitin has few aromatic and basic amino acids (4) which can be detected by uv absorbance or ...

Silver-staining of proteins in polyacrylamide gels: a general overview.

On the basis of the physico-chemical principles underlying silver-staining of proteins, which are recalled in this paper, several methods of silver-staining of proteins after SDS electrophoresis in polyacrylamide gels or isoelectric focusing were tested. The most valuable protocols are presented in this report, including standard methods for unsupported gels and new methods devised for thin (0....

Agarose overlays allow simplified staining of polyacrylamide gels.

Laser scanners from Molecular Dynamics (Sunnyvale, CA, USA) or Hitachi Scientific Instruments (Mountain View, CA, USA) are being used increasingly to detect DNA in polyacrylamide gels. The standard technique for visualizing DNA is to separate the plates of a gel and pour a diluted nucleic acid stain such as ethidium bromide or SYBR Green I [from Molecular Probes (Eugene, OR, USA); also markete...

Visible fluorescent detection of proteins in polyacrylamide gels without staining.

2,2,2-Trichloroethanol (TCE) incorporated into polyacrylamide gels before polymerization provides fluorescent visible detection of proteins in less than 5min of total processing time. The tryptophans in proteins undergo an ultraviolet light-induced reaction with trihalocompounds to produce fluorescence in the visible range so that the protein bands can be visualized on a 300-nm transilluminator...